Plumbagin (1,4-Naphthalenedione, 5-hydroxy-2-methyl-(9CI)) is a natural bicyclic naphthoquinone found in a number of medicinal plants, including Plumbago europaea, Plumbago rosea, Plumbago zeylanica and Dionaea muscipula (venus fly trap). Plumbago family has outstanding record of accomplishment in ayurvedic medicine and traditionally used for microbial infections, cancer, and rheumatoid arthritis (Mossa et al., 2004, Srinivas et al., 2004, Ding et al., 2005 and Hsu et al., 2006) in Asian continent. In specific, plumbagin like naphthoquinones have been used for these treatments. However, most of these claims are supported only by anecdotal evidence, there are few scientific reports describing their cytotoxic efficacy, and literature suggesting their mechanisms is scarce.